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Rat C-peptide EIA背景介紹：

Mercodia Rat C-peptide ELISA provides a method for the quantitative determination of rat C-peptide in serum, EDTA-plasma and cell culture medium.

Rat C-peptide EIA  Summary and explanation of the test

C-peptide is formed together with insulin from the cleavage of proinsulin withinsecretory granules in the ?-cell. In most species the insulin gene exists in a singlecopy. Rats and mice however, have two closely related genes which produce twononallelic proinsulins 1. The rat proinsulins are cleaved to form two insulins (insulinI and insulin II) and two C-peptides (C-peptide I and C-peptide II):The two C-peptides differ with regard to two amino acids in the middle segment ofthe molecule. C-peptide is considered to have a longer half-life in circulation thaninsulin, and is used in humans and animal models as a marker of endogenousinsulin production 2. Traditionally C-peptide has been considered to be withoutbiological effects of its own, but in recent years it has been reported thatC-peptide treatment may affect renal and nerve dysfunction in type 1 diabetespatients 3. Physiological effects of C-peptide have also been observed in animalmodels of diabetes 4,5. Mercodia Rat C-peptide ELISA calibrators are made fromsyntetic rat C-peptide I. Both rat C-peptide I and II are measured in the assay.

Rat C-peptide EIA Principle of the procedure

Mercodia Rat C-peptide ELISA is a solid phase two-site enzyme immunoassay.It is based on the sandwich technique in which two monoclonal antibodies aredirected against separate antigenic determinants on the C-peptide molecule.During incubation, C-peptide in the sample reacts with anti-C-peptide antibodiesbound to the microtitration well. After washing, peroxidase-conjugated antiC-peptide antibodies are added and after the second incubation and a simplewashing step that removes unbound enzyme labeled antibody, the boundconjugate is detected by reaction with 3,3′-5,5′-tetramethylbenzidine (TMB).The reaction is stopped by the addition of acid, giving a colorimetric endpoint that。