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一.產品名稱：Recombinant Murine IL-3/Peprotech Murine IL-3

二.Catalog Number:AF-213-13

三.Synonyms:MCGF (Mast cell growth factor), Multi-CSF, HCGF, P-cell stimulation factor

四.Description:

IL-3 is a hematopoietic growth factor that promotes the survival, differentiation and proliferation of committed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil and mast cell lineages. Produced by T cells, mast cells and eosinophils, IL-3 enhances thrombopoieses, phagocytosis, and antibody-mediated cellular cytotoxicity. Its ability to activate monocytes suggests that IL-3 may have additional immunoregulatory roles. Many of the IL-3 activities depend upon co-stimulation with other cytokines. IL-3 is species-specific, variably glycosylated cytokine. Recombinant murine IL-3 is a 15.1 kDa globular protein containing 135 amino acid residues. Manufactured using all non-animal reagents.

五.AA Sequence:

MDTHRLTRTL NCSSIVKEII GKLPEPELKT DDEGPSLRNK SFRRVNLSKF VESQGEVDPE DRYVIKSNLQ KLNCCLPTSA NDSALPGVFI RDLDDFRKKL RFYMVHLNDL ETVLTSRPPQ PASGSVSPNR GTVEC

六.Source:E.coli

七.Purity:Greater than 98% by SDS-PAGE gel and HPLC analyses.

八.Biological Activity:

The ED₅₀ as determined by the dose-dependent stimulation of the proliferation of mouse NFS-60 cells is ≤0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 10⁷ units/mg.

九.Cross Reactivity:Gerbil, Rat, Mouse